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Adventitious shoots from nodule cluster cultures of Vriesea reitzii: an endemic and endangered bromeliad from atlantic forest Ciência Rural
Rech Filho,Arlindo; Vesco,Lírio Luiz Dal; Guerra,Miguel Pedro.
The Atlantic Forest is a biome with megadiversity and a number of bromeliads take part of it. This is the case of Vriesea reitzii, an endemic bromeliad threatened with extinction. Tissue culture techniques are valuable tools for the mass propagation of bromeliads, thus reducing pressure in the natural habitat. The aim of the present work was to establish an in vitro protocol based on the induction and proliferation of nodule cluster cultures of this species. Plantlets maintained in MS liquid culture medium plus NAA (2µM) and BAP (4µM) had the basal regions of leaves excised and then inoculated in gelled with agar (7g L-1) MS culture medium plus with Dicamba (2.5; 5; 10; 20 e 30µM) and Kin (2µM) or free of plant growth regulators. Nodule cluster cultures...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromeliads; Microshoots; In vitro regeneration; Conservation; Acclimatization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000300043
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Marker-assisted pyramiding of resistance loci to grape downy mildew PAB
Saifert,Luciano; Sánchez-Mora,Fernando David; Assumpção,Wilson Taybar; Zanghelini,Jean Alberto; Giacometti,Renan; Novak,Eduardo Irineu; Vesco,Lírio Luiz Dal; Nodari,Rubens Onofre; Eibach,Rudolf; Welter,Leocir José.
Abstract: The objective of this work was to use a marker-assisted selection for pyramiding the resistance loci Rpv1 and Rpv3.1 in grapevine (Vitis vinifera), and to evaluate their conferred resistance against Brazilian downy mildew (Plasmopara viticola) isolates. A progeny of 23 plants, segregating for the two resistance loci, was obtained by the cross between the Gf 2000-305-122 and Gf.Ga-52-42 genotypes. The progeny was genotyped with four microsatellite markers and phenotyped for resistance to P. viticola using a bioassay with leaf discs. Six plants containing the Rpv1 and Rpv3.1 pyramided loci were identified by the molecular analysis. Plants harboring the Rpv1 + Rpv3.1, Rpv3.1, and Rpv1 loci showed 12.8, 30.0, and 33.1 sporangiophores per leaf disc,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plasmopara viticola; Vitis vinifera; Enological quality; Genetic resistance to disease; Grapevine breeding; Quantitative trait loci.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500602
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